Figure 1

On-chip single-cell-based cell culture system using agarose microchambers. (a) Making of microchambers. Collagen was applied to the chromium-coated glass slides in order to improve the attachment of the cells. After the slides were spin-coated with agarose, microchambers and channels connecting them were formed using a 1064-nm infrared laser beam. (b) On-chip single-cell-based cultivation and observation system.