Figure 10

Microinjection of fluorescence dye into a HeLa cell between synchronized two cardiomyocytes. (a) Phase-contrast image of two cardiomyocytes (black arrowheads) beating synchronizaiton connected by a HeLa cell (black arrow). (b) Fluorescence image of Alexa Fluor 568 Hydrazide (white arrow) injected into the HeLa cell. (c) Phase-contrast image superimposed on the fluorescence image of (b). No dye transfer was found from the HeLa cell to the two cardiomyocytes. (d) Time course of synchronized beating rhythm of two cardiomyocytes connected by a HeLa cell. Bar, 50 μm.