On-chip constructive cell-Network study (I): Contribution of cardiac fibroblasts to cardiomyocyte beating synchronization and community effect

Table 1 Electrical connection of two cardiomyocytes

Connection type*¹	Distance (μm)*²	Delay time (ms)*³	Velocity (m/s)*⁴	N*⁵
CM-CM	60	0.031 ± 0.03	1.8 ± 0.8	12
CM-CM	60	0.059 ± 0.02	1.3 ± 0.7	24
CM-CM	60	0.063 ± 0.03	1.3 ± 0.7	20
CM-CM	60	0.068 ± 0.008	0.90 ± 0.1	60
CM-F-CM	120	0.67 ± 0.03	0.18 ± 0.008	93
CM-F-CM	120	1.5 ± 0.2	0.080 ± 0.01	39
CM-F-CM	120	3.8 ± 0.3	0.032 ± 0.003	95
CM-F-CM	120	6.0 ± 0.6	0.020 ± 0.002	48
CM-F-CM	180	0.91 ± 0.4	0.23 ± 0.08	29
CM-F-CM	180	2.2 ± 0.3	0.085 ± 0.01	50
CM-F-F-CM	180	11 ± 0.4	0.016 ± 0.0006	6

^*1Connection type: CM-CM means two cardiomyocytes directly connection. CM-F-CM means two cardiomyocytes connected by one fibroblast. CM-F-F-CM means two cardiomyocytes connected by two fibroblasts.
^*2Distance: interval of the tips of micropipettes.
^*3Delay time: interval between the action potentials of two cardiomyocytes at 0 mV (mean ± SD).
^*4Velocity: conduction speed (distance/delay time).
^*5N: sampling number of spikes.

ISSN: 1477-3155