Figure 1
From: Thermal Stability of RNA Phage Virus-Like Particles Displaying Foreign Peptides
The plasmids and peptide insertions utilized in this study. (A) pDSP1 expresses the MS2 coat protein single-chain dimer from the T7 promoter. The manipulations that resulted in the various peptide insertions utilized Sal I and Kpn I sites uniquely present in the downstream half of the dimer. The plasmid pET2P7K32 is a similar construction that expresses the PP7 single-chain dimer.