The scheme of the overall AK-based platform preparation and test process. (A) preparation of 1,6-hexane dithiol monolayer. (B) creation of multiple S-S bonds at the ω-position of the monolayer, as a result of oxidation. (C) addition of AK with low concentrations of TCEP for the prevention of aggregate formation. (D) testing the platform, according to the cascade of reactions, presented by Valero et al.