FISH using DOP-PCR products. FISH using DOP-PCR products of the nanodissected microchromosome. Hybridization of the biotinylated probe DNA is detected with FITC-avidin (green signals). Chromosomes and nuclei are counterstained with DAPI (blue). We apply FISH technology as a rapid method for detection of MICs aneuploidy as is clear from the only signal in metaphase.