Effect of p38 or JNK1/2 inhibition on 80 nm- size of SiO
NP induced ATF-2 activation. A549 cells growing on 96-well plates were pre-treated with 10 μM specific inhibitors of p38 (SB202190), or JNK (SP600125) for 1 h and exposed to SiO2NP or anisomycin (positive control) for 24 h. Cells were labelled with the Cellomics® HCS reagent kit for ATF-2 activation. High content screening analysis for nuclear translocation of ATF-2 was performed using an automated IN Cell Analyzer 1000 microscope equipped with IN Cell Investigator image analysis software that quantifies nuclear to cytoplasmic fluorescence intensity. Statistical analysis was carried out by Mann-whitney U test then used to assess the statistical significance differences. p-values of p < 0.05; "**" p < 0.01; "***" for p < 0.001 were considered to be statistically significant.