Figure 8
Effect of labelled 30 nm SiO 2 NP on ATF- translocation at 37°C, under low temperature condition and in the presence of metabolic inhibitor. THP-1 (A), or A549 (B) cells were growing on 96-well plates and exposed to labelled 30 nm SiO2NP (L-SiO2NP) for over 24 h pried at 37°C, 4°C and 0.1% sodium azide. Cells were labelled with the Cellomics® HCS reagent kit for ATF-2 activation. High content screening analysis for nuclear translocation of ATF-2 was performed using an automated IN Cell Analyzer 1000 microscope equipped with IN Cell Investigator image analysis software that quantifies nuclear to cytoplasmic fluorescence intensity. Statistical analysis was carried out by 2-way ANOVA with Bonferroni post-test analysis and statistically significant data is reported by "*" symbol, for p < 0.05; "**" p < 0.01; "***" for p < 0.001.