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Figure 3 | Journal of Nanobiotechnology

Figure 3

From: Profiling and quantitative evaluation of three Nickel-Coated magnetic matrices for purification of recombinant proteins: helpful hints for the optimized nanomagnetisable matrix preparation

Figure 3

Specific binding capacity of three Nickel-magnetic matrices for two His-tagged recombinant proteins. After binding of His-tagged recombinant proteins, His-ProT and His-Mre11, onto the Nickel-magnetic matrices, SiMAC-Nickel, SiMAG/N-NTA/Nickel and SiMAG/CS-NTA/Nickel, flowthrough fractions (FT) were subjected to SDS-PAGE analysis. Percent of band density in FT subtracted from 100% was defined as specific binding capacity. His-ProT (1), His-Mre11 (2), SiMAC-Nickel (A), SiMAG/N-NTA/Nickel (B) and SiMAG/CS-NTA/Nickel (C).

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Journal of Nanobiotechnology

ISSN: 1477-3155

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