Resin type | Protein type | Bead Concentration (mg/ml) | Specific binding capacity (%) | Relative band density (%) | Yield (%) | Recovery (%) | Loss (%) | |||
---|---|---|---|---|---|---|---|---|---|---|
 |  |  |  | E1 | E2 | E3 | E4 |  |  |  |
SiMAC-Nickel | His-ProT | 1 | 68.1 | 11.9 | 10.5 | 10.2 | 10.5 | 43.1 | 63 | 25 |
 |  | 2 | 91.5 | 12.6 | 16.3 | 21.7 | 27.8 | 78.4 | 86 | 13.1 |
 |  | 4 | 95.7 | 1.8 | 3.8 | 11.6 | 64.5 | 81.7 | 85 | 14 |
 |  | 8 | 98 | 0.5 | 0.6 | 1.1 | 66.2 | 68.4 | 70 | 29.6 |
 | His Mre11 | 1 | 45.1 | 2 | 0.1 | 0 | 0.1 | 2.2 | 5 | 42.9 |
 |  | 2 | 46.9 | 2.1 | 0.1 | 0 | 0.1 | 2.3 | 5 | 44.6 |
 |  | 4 | 75.3 | 3 | 0.4 | 0 | 0.1 | 3.5 | 5 | 71.8 |
 |  | 8 | 88.1 | 3.6 | 0.9 | 0 | 0.1 | 4.6 | 5 | 83.5 |
SiMAG/N-NTA/Nickel | His-ProT | 0.5 | 50.4 | 17.8 | 12.8 | 1.6 | 0.4 | 32.6 | 65 | 17.8 |
 |  | 1 | 77.7 | 41.5 | 23.9 | 3.6 | 0.3 | 69.3 | 89 | 8.4 |
 |  | 2 | 82.8 | 29.2 | 37.8 | 9.4 | 1.1 | 77.5 | 93 | 5.3 |
 |  | 4 | 90.1 | 11.8 | 39.2 | 28.3 | 5.4 | 84.7 | 94 | 5.4 |
 |  | 8 | 93.3 | 6.5 | 34.2 | 32 | 7.7 | 80.4 | 86 | 12.9 |
 | His Mre11 | 0.5 | 26.6 | 15.7 | 1.3 | 0.6 | 0.3 | 17.9 | 67 | 8.7 |
 |  | 1 | 27.2 | 16.3 | 1.2 | 1.1 | 0.7 | 19.3 | 71 | 7.9 |
 |  | 2 | 36.4 | 1.3 | 11.4 | 13.6 | 1.2 | 27.5 | 75 | 8.9 |
 |  | 4 | 47.6 | 1.1 | 12.9 | 15.8 | 6.5 | 36.3 | 76 | 11.3 |
 |  | 8 | 50.6 | 0.9 | 2.3 | 10.9 | 5.5 | 19.6 | 38 | 31 |
SiMAG/CS-NTA/Nickel | His-ProT | 0.5 | 11.2 | 0 | 0 | 0 | 0 | 0 | 0 | 11.2 |
 |  | 1 | 17.7 | 3.4 | 0.1 | 0.1 | 0.1 | 3.7 | 21 | 14 |
 |  | 2 | 33 | 5.2 | 3.1 | 1.6 | 1.8 | 11.7 | 35 | 21.3 |
 |  | 4 | 63.4 | 8.6 | 19.1 | 9.2 | 2.5 | 39.4 | 62 | 24 |
 |  | 8 | 65.7 | 12.8 | 14.9 | 3.9 | 1.9 | 33.5 | 51 | 32.2 |
 | His Mre11 | 0.5 | 12 | 0.5 | 1 | 1.2 | 4.1 | 6.8 | 57 | 5.2 |
 |  | 1 | 33.5 | 0.8 | 1.2 | 8.9 | 13.5 | 24.4 | 73 | 9.1 |
 |  | 2 | 46 | 4.1 | 2.6 | 11.7 | 18.5 | 36.9 | 80 | 9.1 |
 |  | 4 | 63.9 | 4.1 | 2.6 | 11.7 | 27.4 | 45.8 | 72 | 18.1 |
 |  | 8 | 65.7 | 4.1 | 2.6 | 11.7 | 27.3 | 45.7 | 69 | 20 |