Figure 8

HIV inhibition of cell associated HIV _IIIB virus infection by HIV-1 gp120 Monoclonal Antibody (F425 B4e8) and Silver Nanoparticles. Chronically HIV-1-infected H9 (10⁵ cells) were incubated with serial two-fold dilutions of HIV-1 gp120 Monoclonal Antibody (F425 B4e8) for 5 minutes with or without silver nanoparticles at 1 mg/mL. Then treated H9 cells were placed into 96-well plates with indicator cells (U373-MAGI-CXCR4_CEM). Assessment of HIV-1 infection was made with a luciferase-based assay after 48 hours. The assay was performed in triplicate; the data points represent the mean, and the solid lines are nonlinear regression curves done with SigmaPlot 10.0 software. By means of Mann- Whitney Rank Sum test we compared the difference in the median values between the two groups (only AgNPs and AgNPs with antibody to HIV-1 gp120 Monoclonal Antibody F425 B4e8) is greater than would be expected by chance; there is a statistically significant difference (P < 0.002).