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Figure 1 | Journal of Nanobiotechnology

Figure 1

From: Optimized labeling of bone marrow mesenchymal cells with superparamagnetic iron oxide nanoparticles and in vivo visualization by magnetic resonance imaging

Figure 1

Representative images demonstrating labeling of MSCs with Feridex in the absence or presence of agents facilitating uptake of the nanoparticles. (A-F") Presence of Feridex in MSCs was detected by Prussian Blue staining or by dextran immunoreactions. (A) Prussian Blue staining in MSCs incubated with FePLL for 24 hrs. Note that virtually all cells display blue intracellular staining. (B-F") Representative images showing dextran immunostaining (green) in MSCs labeled with Feridex and nuclei counterstained with DAPI (blue). (B) Cells incubated with Feridex for 24 hrs in the absence of facilitating agents. (C-F") MSCs exposed to Feridex in the presence of an agent facilitating incorporation (C) FeProt for 4 hrs. (D) FePLL for 24 hrs. (E) FeProt for 24 hrs. (E'-E") Higher-magnification image of the area indicated by the box in (E) illustrating a Feridex-labeled cell whose nucleus is counterstained with DAPI undergoing mitotic division. (E') Mitotic cell (arrow). (E") Merged image showing DAPI and dextran immunostaining. (F-F") Representative images demonstrating the characteristic perinuclear distribution of Feridex in MSCs after 4 hrs of incubation with FeProt (F) DAPI and dextran immunostaining. (F') Brightfield. (F") Merger of the images. Scale bar = 50 μm.

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