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Figure 4 | Journal of Nanobiotechnology

Figure 4

From: Optimized labeling of bone marrow mesenchymal cells with superparamagnetic iron oxide nanoparticles and in vivo visualization by magnetic resonance imaging

Figure 4

Quantitative analysis of the duration of Feridex retention in MSCs pretreated or not with mitomycin C. (A-D) MSCs cultured for up 21 days after initial exposure to FeProt complexes for 4 hrs. (A) Feridex-labeled cells trypsinized weekly and evaluated after 1, 7, 14 and 21 days of culture. (B) The number of MSCs labeled after 1 and 21 days of culture without trypsinization. (C) Mitomycin-pretreated cells labeled with Feridex and trypsinized weekly. The number of MSCs labeled was evaluated after 1, 7, 14 and 21 days of culture. (D) Comparison of the number of MSCs labeled with Feridex after 21 days of culture in the groups illustrated in (A-C). The percentage of labeled cells was significantly higher in FeProt MitC than in the other groups. The "n" indicated on the top of the bars is the number of samples used for the quantification of each time point. Error bars represent SEM. *P < 0.05 and ***P < 0.001.

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