Figure 5
Analysis of the differentiation potential of MSCs labeled with FeProt complexes for 4 hrs. (A-B) Oil Red O staining indicating adipogenesis in unlabeled or Feridex-labeled cells. (A) Unlabeled cells induced toward adipocyte differentiation. (B) Labeled MSCs induced toward adipocyte differentiation. (C-D) Alizarin Red staining showing osteogenic differentiation in MSCs labeled with Feridex or not. (C) Unlabeled cells induced toward osteocyte differentiation. (D) Labeled cells induced toward osteocyte differentiation. (E-F) Alcian Blue staining showing chondrogenesis in unlabeled or Feridex-labeled MSCs. The nuclei were counterstained with Nuclear Fast Red. (E) Unlabeled cells induced to chondrogenic differentiation. (F) Labeled cells induced to chodrogenesis. The brown deposits in figure (F) indicate the presence of SPIONs. No apparent alteration in differentiation potential was observed due to Feridex labeling in MSCs. Scale bar = 50 μm.