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Figure 6 | Journal of Nanobiotechnology

Figure 6

From: Optimized labeling of bone marrow mesenchymal cells with superparamagnetic iron oxide nanoparticles and in vivo visualization by magnetic resonance imaging

Figure 6

Detection of Feridex-labeled MSCs by in vivo and in vitro MRI. (A-C"") Cells labeled with FeProt complexes for 4 or 24 hrs and injected in right or left leg muscles, respectively, were detected by in vivo MRI and by dextran immunofluorescence (A) Representative image of in vivo MRI (transverse plane) showing hypointense (black) spots corresponding to Feridex-labeled cells injected in the leg muscles (white arrows). (B-B"") Dextran immunocytochemistry confirming the presence of Feridex-labeled cells in the right leg muscle. (B) Phase contrast microscopy. (B') Nuclear counterstaining with DAPI. (B") Dextran. (B"') Merged images showing DAPI (blue) and dextran (green) staining. (B"") Merge of images with phase contrast. (C-C"") Dextran immunohistochemistry confirming the presence of Feridex-labeled cells in the left leg muscle. (C) Phase contrast. (C') Nuclear counterstaining with DAPI. (C") Dextran. (C"') Merged images showing DAPI (blue) and dextran (green) staining. (C"") Images merged with phase contrast. Scale bar = 20 μm. (D) In vitro MRI of unlabeled and FeProt labeled MSCs for 4 hrs. As few as 160 cells/μl could be detected by MRI. The "Mean" values are the mean intensities of the gray values in the range of 0-255.

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