Figure 3
Immunofluorescence 24 h after transfection. pIRES-hrGFPII-eIL-12 transfection with the FHD protocol, primary antibody goat IgG anti-p35 and a donkey anti-goat secondary antibody (Texas Red fluorochrome). (a) GFP and Red Fluorescence merged image, (b) GFP Fluorescence and (c) Red Fluorescence images. Scale bar 50 μm. pIRES-hrGFP-HMGB1 transfection with the FHD protocol, primary antibody mouse anti-HMGB1 and secondary antibody goat anti-mouse (Texas Red fluorochrome). (d) GFP and Red Fluorescence merged image, (e) GFP Fluorescence and (f) Red Fluorescence images. Scale bar 75 μm.