Figure 5From: Application of a biotin functionalized QD assay for determining available binding sites on electrospun nanofiber membraneImage of Qdot labeled nanofibers and binding reaction. The fluorescent CLSM image (left) taken shows the uniform attachment of Qdot 655 nm to bound avidin on PVC-COOH nanofibers containing 10%w/w carbon black. The graphical representation (right) show the binding scheme used in the quantification of available biotin binding sites: where avidin is covalently attached to PVC-COOH nanofiber via carbodiimide chemistries (EDC and Sulfo-NHS) and biotin-Qdot complexes bind preferentially to avidin.Back to article page