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Figure 5 | Journal of Nanobiotechnology

Figure 5

From: Application of a biotin functionalized QD assay for determining available binding sites on electrospun nanofiber membrane

Figure 5

Image of Qdot labeled nanofibers and binding reaction. The fluorescent CLSM image (left) taken shows the uniform attachment of Qdot 655 nm to bound avidin on PVC-COOH nanofibers containing 10%w/w carbon black. The graphical representation (right) show the binding scheme used in the quantification of available biotin binding sites: where avidin is covalently attached to PVC-COOH nanofiber via carbodiimide chemistries (EDC and Sulfo-NHS) and biotin-Qdot complexes bind preferentially to avidin.

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