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Figure 3 | Journal of Nanobiotechnology

Figure 3

From: Rapid and specific influenza virus detection by functionalized magnetic nanoparticles and mass spectrometry

Figure 3

Detection sensitivity of HA protein (H5 type) after extraction by Ab H5N2 @Fe 3 O 4 MNP. MALDI mass spectra of (A) protein solution (60 μL) containing HA (200 ng), ribonuclease (1 mg), fetuin (5 mg) and transferrin (5 mg); (B) background peaks from antibody; (C) extraction by AbH5N2@Fe3O4 MNP (HA signals at approximate 75 kDa and 37 kDa, corresponding to singly and doubly charged ions); (D) extraction from a protein solution (60 μL) containing HA (100 ng), ribonuclease (1 mg), fetuin (5 mg) and transferrin (5 mg) by AbH5N2@Fe3O4 MNP; and (E) extraction from a protein solution (60 μL) containing HA (50 ng), ribonuclease (1 mg), fetuin (5 mg) and transferrin (5 mg) by AbH5N2@Fe3O4 MNP. The arrow indicates the experimental m/z of HA. The blue dotted line indicates the antibody. The HA signals after subtraction of the antibody signals are shown in the insets of (D) and (E).

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