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Figure 5 | Journal of Nanobiotechnology

Figure 5

From: Rapid and specific influenza virus detection by functionalized magnetic nanoparticles and mass spectrometry

Figure 5

HA purification performance by Ab H5N2 @Fe 3 O 4 . (A) Lane 1: H5N2 virus (200 ng) prepared from allantoic fluid; Lane 2: isolation of HA protein after incubation of AbH5N2@Fe3O4 MNP with 1 μg of H5N2 virus lysate; Lane 3: isolation of HA protein after incubation of AbH5N2@Fe3O4 MNP with 200 ng of H5N2 virus lysate; Lane 4: control experiment using MEG@MNP for incubation with 200 ng of H5N2 virus lysate; Lane 5: AbH5N2@Fe3O4 MNP only. The antibody signals arise from dissociation of AbH5N2 during SDS-PAGE analysis. (B) Purification efficiency of AbH5N2@Fe3O4 MNP. SDS-PAGE patterns of AbH5N2@Fe3O4 MNP before and after incubation of AbH5N2@Fe3O4 MNP with H5N2 virus lysate in 2 × RIPA buffer. Lane 1: H5N2 virus; Lanes 2-5: incubation of AbH5N2@Fe3O4 MNP (2 μL) with 1, 0.5, 0.1 and 0.01 μL of H5N2 virus, respectively; Lanes 6-9: supernatant of Lanes 2-5.

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