Figure 6

Sequence of the homopurine DNA target. PNA strand bound to double stranded DNA by forming a triplex invasion complex. For better solubility in water, lysine and ethylene glycol linkers were incorporated in the PNA strand. By substitution of cytosine by pseudoisocytosines (J-bases) the pH dependence of the binding process was shifted to a more neutral one. Through the elongated PNA-tail a DNA adapter oligonucleotide can be used for further functionalization purposes - here demonstrated on five repetitive and Cy5 labelled elements.