Cellular protein profiling of biosynthesized SNPs. Biosynthesized silver nanoparticles were gently washed (DW) and equal amount of bound proteins were loaded on reducing SDS-PAGE. The freshly synthesized silver nanoparticles (protein and surfactant free) were incubated with C. reinhardtii cell free extracts for three days. (a) Molecular weight marker (lane no 1), crude extract (lane no 2) and supernatant after nanoparticles synthesis (lane no 3). (b) Silver nanoparticles bound protein after 4 days (lane no 4), 7 days (lane no 5) and 12 days (lane no 6) incubation at reaction condition. (c) Protein binding on pre-synthesized silver nanoparticles of increasing amount 40 μl (lane7), 70 μl (lane 8) and 100 μl (lane 9). The washed isolated nanoparticles were subjected for SDS-PAGE analysis with different increasing volume.