Figure 3
From: Efficiency, error and yield in light-directed maskless synthesis of DNA microarrays
Coupling efficiency vs. time of NPPOC-dT phosphoramidite. Coupling efficiency starts at ~95% at the lowest possible transit time, increases to a maximum and then decreases slowly for long coupling times. Circles indicate a phosphoramidite concentration of 0.03 M. At this concentration and between 30 s and 150 s coupling times, no yield differences can be observed between 1-mers and 12-mers. Squares show coupling efficiency data for the phosphoramidite diluted to 75% of the original concentration. The curve serves as a guide to the eye (lognormal; μ = 52; σ = 6.9; scaled to 100% at the maximum at 80 sec.).