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Table 3 Capping efficiency.

From: Efficiency, error and yield in light-directed maskless synthesis of DNA microarrays

Agent*

Time (s)

Capping efficiency

Acetic anhydride

15

60 ± 4%

Acetic anhydride

30

81 ± 2%

Acetic anhydride

60

92 ± 2%

Acetic anhydride

120

95 ± 2%

DMT-dT

15

91 ± 2%

DMT-dT

30

94 ± 1%

DMT-dT

60

97 ± 1%

  1. *Standard acetic anhydride capping: see Methods and Table 1 for capping protocol. For DMT-dT capping, the concentration was 0.05 M in acetonitrile and applied directly after coupling using the same pulse sequence as NPPOC amidite coupling.
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Journal of Nanobiotechnology

ISSN: 1477-3155

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