Figure 3
From: In vivo characteristics of targeted drug-carrying filamentous bacteriophage nanomedicines
ELISA analysis of anti-phage antibody titer in mice serum. BALB/c mice were immunized with native or with Neo-CAM carrying phages by intravenous (IV) injection. Three doses were evaluated: low (109 phage/dose), medium (1010 phage/dose) and high (1011 phage/dose). The sera samples were collected a week following each injection (weeks 1, 3 and 5), and on the weeks 10 and 22 (n = 5 mice per group). Anti-phage serum titers were determined by ELISA where serial serum dilutions were applied onto phage-coated ELISA plate and detected using HRP-conjugated goat anti mouse antibodies.