Morphological images and genomic DNA apoptosis and Cyclic voltammetry study. (A) Detection of apoptotic and normal cells by Acridine Orange Staining. Apoptotic nuclei could be identified by their distinctively marginated and fragmented appearance. (B) The genomic DNA was isolated from the HCC827 cells that underwent various treatments. The DNA ladders were visualized under UV light. Lane M: Molecular weight markers; Lane 1: Cells treated with 100 μM ruthenium (II)-arene complex; Lane 2: Cells treated with 50 μM ruthenium (II)-arene complex; Lane 3: DNA isolated from HCC827 cells without any treatment. (C) Cyclic voltammetry study of ruthenium (II)-arene complex residue outside HCC827 cells after incubating. (a) ruthenium (II)-arene complex (10 μM); (b) ruthenium (II)-arene complex (10 μM) and cells for 1 h; (c) ruthenium (II)-arene complex (10 μM) and cells for 2 h. Pulse amplitude: 0.05 V; pulse width: 0.05 s; pulse period: 0.2 s.