Figure 4
From: Nanoscaled carborane ruthenium(II)-arene complex inducing lung cancer cells apoptosis
Activation of the apoptosis signal pathway. (A) Western blotting in vitro. Cell lysates were prepared from the cells treated with 50 μM ruthenium (II)-arene complex, 100 μM ruthenium (II)-arene complex. HCC827 cells without treatment were used as a control. The following antibodies were used: anti-cleaved Caspase-8, anti-cleaved Caspase-9, anti-cleaved Caspase-3, and anti-PARP antibody. GAPDH was served as a loading control. (B) Western blotting in vivo. Tumor lysates were prepared from the cells treated with 50 μmol/kg ruthenium (II)-arene complex, 100 μmol/kg ruthenium (II)-arene complex. HCC827 cells without treatment were used as a control. The following antibodies were used: anti-cleaved Caspase-8, anti-cleaved Caspase-9, anti-cleaved Caspase-3, and anti-PARP antibody. GAPDH was served as a loading control.