Figure 6

Typical whole body fluorescence images of the NIR fluorescent P(EF-PLLA) nanoparticles at 5 min (A), 20 min (B), 1 h (C) and 24 h (D) after i.v injection. 12 mice (each experiment group contained 3 mice) were anesthetized and treated with NIR fluorescent P(EF-PLLA) nanparticles (2 mg/mL, 0.01 mg/kg body weight per mouse). Blood was drawn and organs were harvested at each time point. 2 uninjected mice served as negative control. 12 mice were injected correspondingly with free ICG solution, giving similar results (not shown). The experiment was repeated twice with similar results.