The effect of methyl- ? -cyclodextrin (m ? CD) during the protein precipitation. (A) Effect of m? CD on the particle diameter (???) and caspase-3 activation (???) after Cyt c nanoprecipitation. (B) Near-UV CD spectrum of Cyt c nanoprecipitated in absence or presence of m?CD. (C) SEM image of Cyt c NPs made without m?CD (upper picture), and at a 1:8 Cyt c-to-m?CD mass ratio (lower image).