Development of antibody functionalized magnetic nanoparticles for the immunoassay of carcinoembryonic antigen: a feasibility study for clinical use

Table 1 Materials and concentrations used for interference tests for a CEA assay, using the IMR method

Sample no.	Interfering material	Concentration	Mean IMR value(%)	Standard deviation of IMR(%)	pvalue
1	None	-	1.70	0.021	-
2	Hemoglobin	10000 μg/ml	1.71	0.014	0.246
3	Bilirubin	600 μg/ml	1.66	0.021	0.100
4	Triglyceride	30000 μg/ml	1.72	0.007	0.167
5	Uric acid	200 μg/ml	1.69	0.021	0.150
6	Rheumatoid factor	500 IU/ml	1.68	0.014	0.246
7	Intra lipid	30000 μg/ml	1.67	0.007	0.099
8	Albumin	60000 μg/ml	1.67	0.014	0.150
9	Acetaminophen	300 μg/ml	1.72	0.007	0.167
10	Acetyl cysteine	150 μg/ml	1.68	0.021	0.223
11	Acetylsalicylic acid	500 μg/ml	1.74	0.021	0.100
12	Ascorbic acid	300 μg/ml	1.71	0.014	0.246
13	Atrovastatin	3 μg/ml	1.71	0.014	0.246
14	Furosemide	4000 μg/ml	1.70	0.014	0.404
15	Ibuprofen	1000 μg/ml	1.72	0.049	0.326
16	Levodopa	20 μg/ml	1.71	0.014	0.246
17	Methyldopa	200 μg/ml	1.72	0.014	0.150
18	Naprosyn sodium	500 μg/ml	1.66	0.021	0.100
19	Phenylbutazone	400 μg/ml	1.65	0.007	0.052
20	Prednisone	5 μg/ml	1.69	0.014	0.404
21	Tegafur with uracil	50 μg/ml	1.70	0.021	0.500
22	Theophylline	50 μg/ml	1.69	0.014	0.404
23	Warfarin	50 μg/ml	1.66	0.014	0.096
24	Ampicillin sodium	1000 μg/ml	1.67	0.021	0.146
25	Cefoxitin	2500 μg/ml	1.73	0.014	0.096
26	Cyclosporeine A	10 μg/ml	1.71	0.007	0.296
27	Doxycycline hyclate	50 μg/ml	1.68	0.021	0.223
28	Irinotecan	100 μg/ml	1.69	0.014	0.404
29	Lovastatin	2.5 μg/ml	1.72	0.014	0.150
30	Metronidazole	200 μg/ml	1.70	0.021	0.500
31	Oxaliplatin	100 μg/ml	1.72	0.014	0.150
32	Rifampicin	60 μg/ml	1.74	0.007	0.051

The concentration of the CEA in each sample is 5 ng/ml. The matrix is serum. The detected mean value and the standard deviation of each sample are listed. The p values of IMR signals for other samples are calculated using the IMR signals for the pure CEA-serum sample as a reference and the results are listed in the right-most column.

ISSN: 1477-3155