Figure 7
Confocal laser scanning microscopic images of HepG2 cells, with and without TLR4 transfection, treated with TiO 2 NPs. (A) HepG2 cells without transfection and without TiO2 NP exposure, (B) Cells exposed to TiO2 NPs only, (C) Cells transfected with TLR4 expression vector and exposed to 10 μg/ml TiO2 NPs for 48 h. The white arrows show the apoptotic, nuclear fragmented cells. The confocal microscopic images show condensation of chromatin and nuclear fragmentation in HepG2 cells transfected with TLR4 expression vector and exposed to TiO2 NPs.