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Figure 1 | Journal of Nanobiotechnology

Figure 1

From: Characterization of nanoparticle mediated laser transfection by femtosecond laser pulses for applications in molecular medicine

Figure 1

Principle of AuNP mediated laser cell membrane permeabilization. Spherical AuNP were incubated with the cells to allow sedimentation of the particles onto the cell membrane. Prepared samples were placed on an automatized stage to move selected wells of a well plate into the laser focus. Selected wells were completely irradiated by a raster shaped pattern with an inter line distance of 55 μm (1/3 of the laser diameter). A) Side view: the laser beam is weakly focused on the dish bottom where the AuNP labeled cells are located. B) Sketch of manipulation principle: AuNP are in contact with the cell membrane and irradiated by fs-laser pulses (left side). The interaction of the laser pulses with membrane adhered AuNP induces plasmon mediated effects which result in a transient enhanced permeability of the cell membrane. Through this permeabilization, extracellular molecules can cross the cell membrane and diffuse into the cytoplasm (right side). C) By applying a meander shaped scanning pattern, a high number of cells can be treated.

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