Figure 1

Effect of four different chemicals on inflammation-associated insulin signaling pathway in HepG2 cells. The QDot HCS assay was developed for various key kinases involved in inflammation-associated IR that are indicated with a red arrow and validated by specific inhibitors and anti-inflammatory drugs as shown. The p38α, JNK1, and IKKβ involved in the inflammatory pathway and IRS1^ser307, IRS1^tyr, FOXO1, and GSK3β associated with the insulin signaling pathway were simultaneously monitored by QDot multicolor cellular imaging.