Figure 1

Synthesis and characterization of miR-122-loaded graphene-gold composites. A: HRTEM image of miR-122-loaded graphene-gold composites (Scale bar =20 nm). B: Low magnification image of gold nanoparticles (Scale bar =20 nm). C: Confirmed function of miR-122 release by GSH through agarose gel electrophoresis assay; 1) 1 mg/L miR-122, 2) 10 mg/L GGMPN, 3) 10 mg/L GGMPN + 2 μM GSH, 4) 10 mg/L GGMPN + 2 mM GSH, 5) 10 mg/L GGMPN + 10 mM GSH. D: Verified function of miR-122 by GSH through AO fluorescence assay; 1) AO, 2) AO + miR-122, 3) AO + miR-122 + gold nanoparticles, 4) AO + miR-122 + gold nanoparticles + 2 mM GSH, 5) AO + miR-122 + gold nanoparticle + 10 mM GSH. E: TEM image of graphene oxide (Scale bar =50 nm). F: TEM image of GGMPN nanocomplexes (Scale bar =50 nm). G: Protein electrophoresis analysis of P-gp antibody. P-gp antibody (lane 1), P-gp antibody-loaded GGMPN (lane 2). H: Quantification of P-gp antibody remaining in solution before (1) and after 1 h (2) or 24 h (3) exposure to graphene oxide.