Figure 4

Apoptotic assay of HepG2 cells induced by GGMPN. A: HepG2 cells were treated with 10 mg/L GGMPN, and their apoptotic level was detected by AO/EB staining; (a, e, i) image of normal cells, (b, f, j) image of early apoptotic cells, (c, g, k) normal dead cells, (d, h, l) image of late apoptotic cells (Scale bar =10 μm). B: Flow cytometric measurement of cellular apoptosis of resistant HepG2 cells treated with various reagents; a) untreated control, b) transfected cells with miR-122 (1 mg/L, same concentration as loaded on GGMPN), c) treatment of cells with 10 mg/L GGMPN for 36 h, d) quantitative analysis of apoptotic cells after various treatments shown in a), b) and c), *P < 0.05, compared with the control treatment. C: DNA fragmentation in resistant HepG2 cells after different treatments; 1) untreated cells, 2) transfected cells with miR-122 (1 mg/L, same concentration as loaded on GGMPN), 3) treatment of cells with 10 mg/L GGMPN, 4) DNA marker. D: Western blot analysis of activated Caspase levels after various treatments; 1) without treatment cells, 2) cells transfected with miR-122, 3) cells treated with GGMPN.