Figure 5
Photothermal therapy assay of HepG2 cells treated with GGMPN. A: Images of photothermal therapy of cells with various treatments; a) control experiment, b) transfected with miR-122, c) treated 10 mg/L GGMPN with laser power threshold of 20 W/cm2 for 1 min, d) or 10 min. (Scale bar = 20 μm). B: Photo of photothermal therapy with semiconductor laser light. C: Quantitative analysis of apoptotic cells after various treatments as shown in A, * P < 0.05, compared to the control treatment. D: Laser hyperthermia with laser irradiation light for inhibition of tumor growth in HepG2 nude mice with different treatments, a) untreated used as control, b) transfected with miR-122 (1 mg/kg, same concentration as loaded on GGMPN), c) treated 10 mg/L GGMPN with laser power threshold of 2 W/cm2. E: Photo of tumor irradiated for by laser hyperthermia; a) photo of laser hyperthermia, b) untreated control, c) tumor irradiated by laser hyperthermia. F: Quantitative analysis of tumor volume after various treatments as in (D). G: Western blot analysis of apoptosis-related protein in tumors of mice treated as in (D).