Figure 7

Cytotoxic studies. (A) Cell morphology by neutral red and Hoechst-33258 staining. (a-a’) Control cells. (b-b’) Cells incubated with DMSA-SPION for 24 h. Scale bar represents 10 μm. (B) Cell cycle analysis of control (untreated) and cells incubated with SPION for 24 h. (C) Analysis of ROS generation by DCFH-DA assay. Cells incubated with DMSA-SPION for different times and loaded with DCFH-DA were visualized under bright field or fluorescence microscopy, respectively. (a, a’) Cells incubated with nanoparticles for 24 h. (b, b’) Cells incubated for 48 h. (c-c’) Cells incubated for 72 h. Scale bar represents 50 μm. (D) Cytotoxicity analysis in MCF-7 cells incubated DMSA-SPION for 24 h. (a) MTT cell viability assay after 24 h of treatment with 0.05, 0.1 and 0.4 mg ml⁻¹. (b) Trypan blue exclusion test immediately after incubation at 0.4 mg ml⁻¹.