Figure 3

Effects of MNPs on cell cycle. hVM cells were either untreated or treated with 50 μg/ml of 100 nm MNPs for 24 and 48 h. Cell cycle analyses were performed by flow cytometry after staining with propidium iodide (PI). The intensity of the PI signal is directly proportional to the DNA content in each phase (G0-G1 phase, S phase, G2-M phase). The percentage of cells in each cell cycle phase showed similar cell cycles profiles for labeled and unlabeled cells.