Figure 4

Effects of MNPs on cell stemness and differentiation. A. Percentage of nestin positive hVM cells after labeling cells with 50 and 100 nm MNPs at 50 μg/ml for 72 h. B. The presence of neurons (β-III-Tubulin+), dopaminergic neurons (TH+) and astrocytes (GFAP+) cells was assessed in hVM cells differentiated for 7 days after labeling them with 50 and 100 nm MNPs at 50 μg/ml for 72 h. C. Immunocytochemistry for β-III-Tubulin, TH and GFAP in 7-day differentiated hVM cells either unlabeled (control) or labeled with 100 nm MNPs-Cy3 (in red). Nuclei were counterstained with ToPro3. No significant differences were observed in the relative proportions of the different neural cell types or in their morphology in MNPs-labeled cells with respect to unlabeled cells. Scale bar: 25 μm.