Figure 8
MR imaging of hVM-MNP labeled cells 5 months after transplantation into hemiparkinsonian rat brains. hVM-MNP-Cy3 labeled cells (3x105 cells) were detected by MRI 5 months (arrow in A) after transplantation into the right striatum of 6OHDA-lesioned rat brains. Analyses by fluorescence microscopy showed that MNPs-Cy3 were clearly visible in red in coronal sections from striatum 5 months after cell transplantation (B, D). Transplants were stained for hNu (blue in C) to detect hVM cells; Nestin (red in C and insert in C); human GFAP (blue in D and E, orthogonal projections are shown in E, confirming the colocalization of GFAP and MNPs-Cy3) and OX42 (stained with DAB) combined with red fluorescence to visualize MNPs-Cy3 (F). Scale bars: 2 mm in A, 100 μm in B-D; 10 μm in insert in C, E-F.