Figure 7
Real-time polymerase chain reaction: Activity of X-linked inhibitor of apoptosis, A20, Bcl-2, Bcl-xL, caspase-3 and PARP-1were examined. The relative activities of A20, Bcl-2, Bcl-xL and caspase3 in HCT116 cells treated with ι-car-γ- Fe2O3 nanocomposite for 24 h were higher than control cells. β-actin was examined as an endogenous control. Significant difference is observed between control and XIAP (p < 0.01), A20 (P < 0.05), Bcl-2 (p < 0.01), Bcl-xL (p < 0.01), caspase3 (p < 0.05) and PARP-1 (p < 0.01). *- p < 0.05, **-p < 0.01.