Figure 4

Internalization of albumin nanoparticles. Internalization of albumin nanoparticles was examined by confocal laser scanning microscope. SKOV3 cells were incubated with alexa-488 conjugated BSA-ABZ 10 nm (A) and Nab-ABZ 200 nm (B) and the nucleus was stained with PI (red). The fluorescence intensity and the cell images were obtained using two channels: green λ_ex/ λ_em = 488 nm/ 492–508 nm for alexa-488 conjugated BSA NPs and red ( λ_ex/ λ_{em =} 568 nm/ 612–622 nm) for the PI. Scale bar is 50 μm and magnification 60Χ with oil immersion lenses. Cells were treated with fluorescent nanoparticles for 15, 30 and 60 min and uptake of BSA-ABZ 10 nm (C) and Nab-ABZ 200 nm (D) was quantitatively measured by FACS analysis. Right shift of the chromatogram indicates more cellular uptake (C). Relative fluorescence intensity was also measured by FACS analysis (E). Data are presented as mean ± SD (n = 2).