Skip to main content
Figure 1 | Journal of Nanobiotechnology

Figure 1

From: Streptavidin functionalized polymer nanodots fabricated by visible light lithography

Figure 1

Incubation of nanodots. a) SEM image of TPP fabricated nanodots with a surface area of 1×105-2×105 nm2, polymerized with Irgacure 819 photoinitiator. b) Nanodots incubated with Alexa555-streptavidin (@ 532 nm excitation). c) Nanodots with streptavidin after additional Atto655-biotin incubation (@647 nm excitation). All images were taken with 10 ms illumination time, no Irgacure 819 autofluorescence was detected for such a short time interval. d) A sketch of the incubation process for the nanodots. First phosphate buffered saline (PBS) solution with lipid vesicles is added. After the incubation, the slide is washed with PBS and Alexa555-streptavidin dilution is added. Subsequently after an additional PBS washing step, we add Atto655-biotin diluted in PBS.

Back to article page