Schematic drawing of nanostructured dots. a) Sketch of nanostructured dots (nanoanchors) fabricated via STED-lithography and incubated with fluorescent streptavidin. Only 10% of all dots were covered with proteins. b) Sketch of TPP fabricated dots having ~30% average streptavidin loading per dot. c) and d) are sketches of the largest fabricated dots with over 80% and 100% streptavidin coverage.