Figure 7
Effect of free drug and drug loaded NPs on the cell cycle distribution of MG63 cells. For cell cycle analysis, cells were treated with respective formulation and 24 h post treatment, stained with PI and RNAse before flow cytometer analysis. The region marked M1, M2, M3 and M4 represent G1, S, G2/M and G0 phase respectively, of the cell cycle. **p < 0.01 is the statistical difference between the combinational nanoparticles and individual free drugs.