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Figure 5 | Journal of Nanobiotechnology

Figure 5

From: Design and characterization of a new peptide vector for short interfering RNA delivery

Figure 5

a Fluorescence microscope image of GL1-siRNA complexes at molar ratio 40/1 (magnification 40×). The red fluorescence indicated Cy3 labeled siRNA, blue fluorescence represented DAPI stained nuclei. The lower panel was non-treated cells, used as a control. b FACS results of cellular uptake of siRNA. *P value < 0.05, in a serum free environment, the uptake of siRNA at molar ratios of 20/1 and 40/1 is significantly different from that after Lipo treatment. **P value < 0.05, in a serum environment, the uptake of siRNA at a molar ratio of 20/1 is significantly different from that at a molar ratio of 40/1 and after Lipo treatment. c Relative fluorescent intensities at different treatment conditions. *P value < 0.05, the fluorescence intensity at molar ratios of 20/1 and 40/1 with or without serum is significantly different from that after the other treatments. Non-treated sample was negative control; Lipo-siRNA complexes were positive control. Cy3 labeled GAPDH siRNA was used here. siRNA concentration was 100 nM in both experiments. NT non-treated, MR peptide/siRNA molar ratio, Lipo Lipofectamine 2000. Results are expressed as mean ± standard deviation (n = 3).

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