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Figure 7 | Journal of Nanobiotechnology

Figure 7

From: Nanohydroxyapatite-reinforced chitosan composite hydrogel for bone tissue repair in vitro and in vivo

Figure 7

Effect of Zn-CS/nHAp/β-GP hydrogel on the expression of Runx2 protein. mMSCs were grown in the presence of Zn-CS/β-GP hydrogel or Zn-CS/nHAp/β-GP hydrogel under normal and osteogenic conditions for a 7 days and c 14 days. At the end of the incubation periods, whole cells lysates were prepared and separated onto 12% SDS-PAGE. The proteins were electrophoretically transferred to PVDF membranes and subjected to western blot analysis using Runx2 antibodies. Cdk2 antibodies were used as an internal loading control. b and d represent the quantification of Runx2 protein blots as mentioned above using Image Lab software version 4.1 (Bio-Rad). NM normal medium, OM osteogenic medium.

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