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Figure 2 | Journal of Nanobiotechnology

Figure 2

From: Recombinant Hepatitis E virus like particles can function as RNA nanocarriers

Figure 2

A Schematic representation of in vitro transcribed RNA constructs. a 5′-methyl-G-RFP, b 5′-methyl-G-HBsAg, c 5′-methyl-G-HEV-(1249 nt)-RFP, d 5′-methyl-G-HEV-(1249 nt)-HBsAg, e 5′-methyl-G-HEV-(199444 nt)-RFP, f 5′-methyl-G-HEV-(199444 nt)-HBsAg. B Urea gel representing integrity of various in vitro transcribed RNAs (with 5′ cap and 3′ poly-A tail). Lane 1 5′-methyl-G-HEV-(1249 nt)-RFP (947 bp); lane 2 5′-methyl-G-RFP (698 bp); lane 3 5′-methyl-G-HBsAg, (677 bp); lane 4 5′-methyl-G-HEV-(1249 nt)-HBsAg (926 bp); lane M RNA Millenium marker (Ambion). The size of all above transcribed RNAs in gel, seems greater by 30–50 nt, due to 3′ poly-A tail added to them. C Urea gel representing integrity of various in vitro transcribed RNAs (with 5′ cap and 3′ poly-A tail). Lane 1 5′-methyl-G-HEV-(199444 nt)-RFP (943 bp); lane 2 5′-methyl-G-HEV-(199444 nt)-HBsAg, (922 bp); lane M RNA Millenium marker (Ambion). The size of all above transcribed RNAs in gel, seems greater by 30–50 nt, due to 3′ poly-A tail added to them.

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