Figure 4

a Humoral response (against HBsAg antigen) raised in 5′-methyl-G-HEV-(1–249 nt)-HBsAg-pORF2 VLP complex immunized mice. The anti-HBsAg antibodies (quantitated by ELISA) were observed in Balb/c mice immunized with 5′-methyl-G-HEV (1–249 nt)-HBsAg RNA-pORF2 VLP comlpex. Asterisks show significant increase (**p < 0.01) in antibody titre (41st day post-immunization) as compared to un-immunized control mice. All the statistical analyses were done by multiple t test. b Humoral response (against HEV pORF2) raised in 5′-methyl-G-HEV-(1–249 nt)-HBsAg-pORF2 VLP complex immunized mice. The anti-pORF2 antibodies (quantitated by ELISA) were observed in Balb/c mice immunized with 5′-methyl-G-HEV (1–249 nt)-HBsAg RNA-pORF2 VLP complex. Asterisks show significant increase (**p < 0.01) in antibody titre (48th day post-immunization) as compared to un-immunized control mice. All the statistical analyses were done by multiple t test.