Fig. 3

Orthogonal view from different planes (x/y, x/z or y/z) of the confocal microscope images used to analyze the particle uptake. Examples correspond to: a “small” ellipsoidal CaCO₃ particles and b “big” spherical SiO₂ particles (used as control). Co-localization of fluorescently labeled CaCO₃ particles (with TRITC, in red) with the lysosomal marker Anti-LAMP1 labeled with DyLight 649 (artificially colored in yellow). The HeLa cell´s nucleus was stained with DAPI (in blue) and the cytoskeleton with Oregon Green^® 488 phalloidin (in green). Scale bars correspond to 20 µm.