Fig. 4

The injection time of Hepcidin-25 into the MIP NP SPR sensor. Different injection volumes of Hepcidin-25 were tested (from 30 to 300 µl) at a constant flow rate of 30 µl/min allowing the contact time between the ligand and the analyte to vary from 1 to 10 min (a 1 min, b 3 min, c 5 min, d 10 min). An injection volume of 90 µl (3 min) b was chosen for all the experiments